Central precocious puberty patients’ enrollment and the proteomic profile

A total of 15 girls hospitalized with precocious puberty were enrolled from the Department of Pediatrics, the First Affiliated Hospital, College of Medicine, Zhejiang University in 2020. Central precocious puberty was confirmed according to the diagnostic criteria of CPP. The mainly inclusion criteria were as follows: 1. The chronological age was less than 8 years old and breast developed more than six months. 2. The bone age exceeded chronological age more than 1 year. 3. Ovarian volume greater than 1.0 ml. 4. The peak luteinizing hormone (LH_peak) is more than 5 mIU/ml and LH_peak/FSH_peak is more than 0.6 by LHRH test. 5. Organic central precocious puberty was excluded. Eventually, 15 girls diagnosed as central precocious puberty were qualified. Their mean age is 7.06 ± 1.37 years and bone age are 9.27 ± 0.31 years. BMI is 15.03 ± 1.27 kg/m^². Luteinizing hormone (LH) at base line is 0.93 ± 0.33 mIU/ml and the peak LH is 18.48 ± 5.78 mIU/ml. Follicle-stimulating hormone (FSH) at base line is 3.14 ± 0.39 mIU/ml and the peak of FSH is 15.30 ± 1.25 mIU/ml. The LH_peak/FSH_peak ratio is 1.17 ± 0.30. The left ovary volume is 2.45 ± 0.36 ml and the right is 2.22 ± 0.28 ml. 15 healthy girls were enrolled as normal group. Neither their parents nor their families had a history of precocious puberty. They were all from the Hailiang Primary School in Zhuji, Zhejiang Province, China. Their mean age was 7.19 ± 0.86 years and had no precocious puberty or puberty (Breast development, B1 phase) and BMI is 16.78 ± 1.48 kg/m². The research protocol conformed to the ethical guidelines of the Declaration of Helsinki was approved by Clinical Research Ethics Committee of the First Affiliated Hospital, College of Medicine, Zhejiang University (No. 2020-300). To analyze the global protein expression atlas in central precocious puberty (CPP group) and healthy girls (Normal group), we collected the plasma and utilized LC-MS/MS to analyze the sample following the workflow described in Figure 1. As we known, our data firstly mapped the proteomic profile in central precocious puberty.

The workflow of proteomic analysis between normal and CPP group.