Measurement of wound dimensions

Wounds were photographed using a fixed focal length lens to ensure the same point of view and scale by the same study team member. To have a correct scale indicator, a commercially available industrial photographic colour standard table was photographed along with the wounds. It was used both to set correct colours of the image and to measure the wound. When the wound was too big to fit on a single image, several individual images were taken around the wound to have a good view for wound surface measurement. Wound measurements were conducted using photographic software by counting pixels within the wound borders. As a reference, the number of pixels inside a 1 cm² square was used. The 1 cm² square was photographed for each image. If the wound was too big to fit on a single picture, all fragments on successive pictures were summed.