Etiolated hypocotyls were dipped in 10 μg/mL propidium iodide (PI) in H2O for 20 min in the dark, rinsed twice with H2O for 1 min, and the PI fluorescence was visualized using a 20x objective on a Zeiss LSM880 confocal laser scanning microscope. For cryo-SEM, hypocotyls were frozen in liquid nitrogen before imaging. Images were obtained using a FEI Quanta 200 scanning electron microscope with cryo system (Quorum PP2000TR FEI) operating at an acceleration voltage of 3 kV. For cell size measurement, 30 cells of at least 10 etiolated hypocotyls for each treatment were used and processed using the ImageJ software. Statistical analysis was performed using the GraphPad Prism software version 8.0 for Mac.
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