The Hi-C matrices were exported with HiCExplorer (v3.6) to h5 format. The scaffolds for each species were classified as either macro- or microchromosomes (SI Appendix, Table S2). Using the hicAdjustMatrix function, independent matrices were created for macrochromosomes and microchromosomes. Distance-dependent contact probabilities [P(s)] were calculated using hicPlotDistVsCounts (from the HiCExplorer package), which were plotted with a maximum distance of 1 × 108 bp.
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