Annexin-V assay

Annexin V assays were performed using an Annexin V/FITC kit from Abcam (ab14085) following the manufacturer's instructions. Briefly, cells were collected and counted. Then, 1 × 10⁵ cells were mixed with 500 μl Annexin V binding buffer, 5 μl Annexin V-FITC and 5 μl propidium iodide. The mixture was incubated for 5 mins at room temperature in the dark. The Annexin V-FITC signal was then analysed via flow cytometry (Ex=488 nm, Em=530 nm) using a FITC signal detector and a phycoerythrin emission signal detector to detect PI staining.