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Cold Plate Test

LM Laura Micheli
RR Raghavan Rajagopalan
EL Elena Lucarini
AT Alessandra Toti
CP Carmen Parisio
DC Donatello Carrino
AP Alessandra Pacini
CG Carla Ghelardini
PR Parthasarathi Rajagopalan
LM Lorenzo Di Cesare Mannelli
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Thermal allodynia was assessed using the cold plate test. With minimal animal-handler interaction, rats were taken from home-cages, and placed onto the surface of the cold-plate (Ugo Basile, Varese, Italy) maintained at a constant temperature of 4 ± 1 °C. Ambulation was restricted by a cylindrical Plexiglas chamber (diameter 10 cm, height 15 cm), with open top. A timer controlled by foot pedal began timing response latency from the moment the mouse was placed onto the cold plate. Pain-related behavior (licking of the hind paw) was determined by recording the time (seconds) of the first sign of licking of the hind paw. The cutoff time of the latency of paw lifting or licking was set at 30 s [24, 25].

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