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Experimental mice were allowed to freely explore a new home cage (L: 39.1 cm × W:19.9 cm × H:16 cm) for 5 min (habituation), before introduction of an age-matched novel conspecific (female or male). Social interaction was then monitored during two contiguous epochs (lasting 3 min each), one of which was paired with photostimulation (473 nm; 20 Hz, 10 ms pulses; 5–8 mW, 3 min) in a counterbalanced order. Time interacting was manually scored by a trained observer blind to experimental conditions.
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