2.2. Next‐generation sequencing analysis sequencing and single‐nucleotide polymorphism analysis

The fresh conidial spores of isolate 415‐2 were inoculated into liquid MM and shaken for 24 h at 37°C at 200 rpm, and the resulting mycelial pellets were dried and extracted to obtain genome DNA (gDNA). The next‐generation sequencing (NGS) experiment was performed at Shanghai OE Biotechnology Co., Ltd., as a commercial service. gDNA of 415‐2 was sequenced by using the Illumina HiSeq 2000 platform with 100‐bp paired‐end reads in a high‐output mode. An average depth of each nucleotide was gained. Sequence assembly and mapping were referred to the A. fumigatus A1163 genome (http://www.ncbi.nlm.nih.gov/assembly/GCA_000150145.1). Analysis of mapping quality and SNPs was performed by using a next‐generation sequencing data analysis suite, SHORE software.