2.1. Genomic Data Preparation of Ischemic Stroke

The current study uses IS gene expression data sourced from the GEO's genomic database. Gene annotation was extracted with the resultant data being retrieved such as Entrez ID as well as gene symbols and based on an inherent reference to the platform {"type":"entrez-geo","attrs":{"text":"GPL570","term_id":"570"}}GPL570 (Illumina Inc.). In an effort to ensure the comparability and integrity of the selected data sets, a calculation of normalization with log 2 manifestation is made with RMA package. The mapping of the gene annotation in the identical gene symbol corresponding to multiple probes is made with the probe with the biggest mean manifestation across all samples. Consequently, the expression matrix of the samples is mixed up based on the miRNA/gene symbols. All the samples are then tested in an effort to alleviate the batch effect through reliance on the SVA toolkit.