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Purification and stability of recombinant GFP
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Recombinant GST-GFP was obtained by affinity chromatography as a glutathione-S-transferase (GST-GFP) fusion protein from the soluble fraction of E. coli XL1 Blue harboring plasmid pGEX-GFP. Endotoxicity of the purified GST-GFP was determined by Limulus lysate assay (Sigma-Aldrich), following product information technical bulletin. The stability and integrity of GST-GFP were tested as described previously [37].
Copyright and License information: ©2021 Chacón-Díaz et al
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