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Cell viability assay

AA Amber Anees
HS Hannah E. Salapa
PT Patricia A. Thibault
CH Catherine Hutchinson
SH S. Austin Hammond
ML Michael C. Levin
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Neuro-2a cells were plated onto poly-D-lysine coated 96-well plates. The CYQUANT lactate dehydrogenase assay (LDH; Fisher Scientific) was performed according to the manufacturer’s protocol to assess overall cell viability in differentiated Neuro-2a cells treated with siNEG or siA1 for 72 h. Three experimental replicates were performed with each condition performed in triplicate. Absorbance was measured at 680 and 490 nm using a spectrophotometer microplate reader. LDH activity was calculated through the subtraction of the absorbance at 680 nm from the absorbance at 490 nm. Percent cytotoxicity was calculated using the following formula:

Copyright and License information:  ©2021 Anees et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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