Stool sample treatment and nucleic acid extraction

HL Huanhuan Lu
JX Jinbo Xiao
KZ Keyi Zhang
ZH Zhenzhi Han
YS Yang Song
DW Dongyan Wang
TJ Tianjiao Ji
DY Dongmei Yan
SZ Shuangli Zhu
WX Wenbo Xu
YZ Yong Zhang
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All the fecal samples were intended for nucleic acid extraction; therefore, they required special handling. The stool samples (approximately 2 g each) were dissolved in 10 mL of phosphate-buffered saline containing antibiotics and thereafter treated with 1 g of glass beads and 1 mL of chloroform, followed by vigorous shaking for 20 min using a mechanical shaker and centrifugation for 20 min at 1500 × g in a refrigerated centrifuge. Then, the supernatant was stored at − 80 °C until further analysis. RNA was extracted from the stool suspension using a QIAamp Viral RNA Mini Kit (Qiagen, Hilden, Germany).

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