All the fecal samples were intended for nucleic acid extraction; therefore, they required special handling. The stool samples (approximately 2 g each) were dissolved in 10 mL of phosphate-buffered saline containing antibiotics and thereafter treated with 1 g of glass beads and 1 mL of chloroform, followed by vigorous shaking for 20 min using a mechanical shaker and centrifugation for 20 min at 1500 × g in a refrigerated centrifuge. Then, the supernatant was stored at − 80 °C until further analysis. RNA was extracted from the stool suspension using a QIAamp Viral RNA Mini Kit (Qiagen, Hilden, Germany).
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