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The cells were plated in a 60 mm plate and transfected with the pDR-GFP reporter alone (as negative control) or together with the pCAGGS-I-SceI plasmid, by the FuGene HD (Promega) reagent. After 48 hours cells were collected and analyzed by FACS analysis with BD Accuri C6 Flow Cytometer (BD Bioscience, Canada).
Copyright and License information: ©2021 The Authors. Published by Elsevier Ltd.
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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