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Human pancreatic cancer cell lines, PANC-1 and BxPC-3 were purchased from the American Type Culture Collection (Manassas, VA, USA). PANC-1 cells were maintained in Dulbecco’s Modified Eagle’s Media (DMEM) (Sigma-Aldrich, St Louis, MO, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Sigma-Aldrich, St Louis, MO, USA). BxPC-3 cells were maintained in RPMI-1640 medium (Sigma-Aldrich, St Louis, MO, USA) containing 10% FBS. Both cell lines were grown in an incubator in 5% CO2 at 37 °C.
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