2.8. Detection of VEGFA Protein Concentration

To investigate the pathological mechanisms under hyperglycemia, the supernatant of the different groups was harvested and centrifuged at 4°C for 10 min at 3000 × g to remove the cell debris, and then it was stored at -80°C for ELISA. After dilution, the concentration of VEGFA in cell supernatants was quantitatively detected using the Human VEGFA ELISA kit (RayBiotech Systems, USA) following the manufacturer's instruction. Data was quantified in comparison to VEGF standards.