Materials

Dopamine hydrochloride, ammonia aqueous solution (NH₄OH, 28–30 wt%), ethanol, pluronic F127, 1, 3, 5-trimethylbenzene, and IR-780 were purchased from Sigma-Aldrich (Shanghai, China). Mouse breast cancer cells (4T1) were purchased from American Type Culture Collection (ATCC, Manassas, VA). CD3, CD4, CD8 flow cytometric antibodies and their homotypic antibodies were obtained from BD Biosciences (Shanghai, China). Anti-calreticulin (CRT) antibody was obtained from Abcam (Shanghai, China). The enzyme linked immunosorbent assay (ELISA) kits for the detection of adenosine triphosphate (ATP), high-mobility group box 1 (HMGB1), tumor necrosis factor (TNF-α), interferon γ (IFN-γ), interleukin-2 (IL-2), and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) were obtained from KeyGEN Biotech. Co., Ltd. (Jiangsu, China). Tumor dissociation kit of mouse was purchased from Miltenyi Biotec (Germany). Fetal bovine serum (FBS), trypsin, Roswell Park Memorial Institute 1640 (RPMI 1640) culture medium, and phosphate buffer saline (PBS) were purchased from Gibco/Life Technologies (Shanghai, China). Singlet oxygen sensor green (SOSG) kits were purchased from Invitrogen (Shanghai, China).