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Establishment of the Drug-Resistant Cell Line
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The HCC827 cells were cultured in a medium containing afatinib (Selleck Chemicals, Houston, TX, USA)at 1 nmol/L. When the cells showed viability similar to that of cells without afatinib, we gradually increased the concentration of afatinib until it reached 6 μmol/L. The entire induction period lasted approximately six months.
Copyright and License information: ©2021 Zhu, Zhou, Lu, Zhang, Chen and Li
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