RNA isolation and real‐time PCR analysis

Total RNA was isolated following the manufacturer’s instructions (TRIzol, Invitrogen). RNA was reversely transcribed with the iScript cDNA Synthesis Kit (Bio‐Rad). Real‐time PCR analysis was performed with the KAPA SYBR FAST qPCR MasterMix (Peqlab) on an iCycler IQ system (Bio‐Rad). Relative expression levels were normalized to HPRT (mouse) or B2 M (human).

Hprt forward (5′) and reverse (3`) (GCTGGTGAAAAGGACCTCT and CACAGGACTAGAACACCTGC Cxcl1 forward and reverse (CTG CAC CCA AAC CGA AGT C and AGC TTC AGG GTC AAG GCA AG); Cxcl10 forward and reverse (TTC TGC CTT CAT CCT GCT G and AGA CAT CTC TGC TCA TCA TTC); Cldn1 forward and reverse (CTT CTC TGG GAT GGA TCG and GGG TTG CCT GCA AAG TAC TGT); Cldn3 forward and reverse (GCG GCT CTG CTC ACC TTA GT and GAC GTA GTC CTT GCG GTC GTA); Cldn8 forward and reverse (TCA GAA TGC AGT GCA AGG TC and AGC CGG TGA TGA AGA AGA TG), Cdkn2a forward and reverse (GAA CTC TTT CGG TCG TAC CCC and CAG TTC GAA TCT GCA CCG TAG); Cdkn2b forward and reverse (CCC TGC CAC CCT TAC CAG A and CCC TGC CAC CCT TAC CAG A); Isg15 forward and reverse (AAG CAG ACT CCT TAA TTC C and CTG TAC CAC TAG CAT CAC); Tnf forward and reverse (CAG GAG GGA GAA CAG AAA C and ACA AGC AGG AAT GAG AAG AG); Oasl1 forward and reverse (ACC AGC AGT ATG TGA GAG and GGT CCA GTA GAT ACA GAG G); Ccr2 forward and reverse (ACG ATG ATG GTG AGC CTT GTC and CAG GAA GAG CAG GTC AGA GAT G); IAP forwards and reverse (ACT AAC TCC TGC TGA CTG G and TGT GGC TTG CTC ATA GAT TAG); Dnmt1ex3‐4 forward and reverse (GAG GAA GGC TAC CTG GCT AAA G and TCA CTG TCC GAC TTG CTT CTC); MLV forward and reverse (CCA GAC TTG ATC CTG CTA CA and CTC AGT CAG CCA TCT CTG AC); LV30 forward and reverse (CTC GCA CCT TTT CGC GCT CG and GCG AGG GGA TCA TCA TAA CA); Ccl5 forward and reverse (ATA TGG CTC GGA CAC CAC TC and CCC ACT TCT TCT CTG GGT TG); Mavs forward and reverse (GGA CAC ACT CTG GGG ACT CT and GGT CAG GGA TGT TGT GAC CT); Ifit1 forward and reverse (GCT CTG CTG AAA ACC CAG AG and CCC AAT GGG TTC TTG ATG TC); Ccl5 forward and reverse (CCC TCA CCA TCA TCC TCA CT and GAG CAC TTG CTG CTG GTG TA); Aim2 forward and reverse (CCT GAT TCA AAG TGC AGG TG and GAG GCA GCA GAG CAG TTT TC); Il1b forward and reverse (ACT CAT TGT GGC TGT GGA GA and TTG TTC ATC TCG GAG CCT GT); Il6 forward and reverse (GAG GAT ACC ACT CCC AAC AGA CC and AAG TGC ATC ATC GTT GTT CAT ACA); Ifna forward and reverse (CGC AGG AGA AGG TGG ATG and TGC TGG TGG AGG TCA TTG); Ifng forward and reverse (GCT GAC CTA GAG AAG ACA CAT C and TTC CAC ATC TAT GCC ACT TGA G).

B2M forward and reverse (GAG TAT GCC TGC CGT GTG AAC and TCT AAG TTG CCA GCC CTC CTA G); CCL5 forward and reverse (CTG CTT TGC CTA CAT TGC CC and TCG GGT GAC AAA GAC GAC TG); IFT2 forward and reverse (CAA AGG GCA AAA CGA GGC AG and CCC AGG CAT AGT TTC CCC AG).