Animals

All animal work was performed and approved by the Institutional Animal Care and Use Committee (IACUC) of Tongji Medical College, Huazhong University of Science and Technology. All mice were housed in the specific pathogen-free animal facility of Huazhong University of Science and Technology. Floxed Hnrnpu transgenic mice (Hnrnpu^flox/flox) were a gift from Dr. Tom Maniatis's lab at Columbia University. The Amh-Cre deletor mice were purchased from Jackson Laboratory and maintained on the C57BL/6J background. To obtain Amh-Cre; Hnrnpu^flox/flox (designated as Amh-Cre-cKO) male mice, Amh-Cre males were mated with Hnrnpu^flox/flox females initially to generate the Amh-Cre; Hnrnpu^+/flox. Then they were bred with Hnrnpu^flox/flox mice to generate Amh-Cre-cKO males. The genotyping strategy of Hnrnpu-floxed mice was performed as the previous report ³¹.