DNA Sequence Analysis of the gyrB and acdS Genes

The acdS and gyrB gene sequences were retrieved from the annotated genomes and imported into Geneious. Sequences for both gyrB and acdS genes were multi-aligned using the software MUSCLE and subsequently trimmed to result in a sequence length of 879 bp for each of the gyrB sequences and 1,150 bp for the acdS sequences. Before phylogenetic construction, the J model test (2.1.10) was used to determine the best model fit for the alignment. Separate maximum likelihood trees for both the gyrB and acdS gene were then constructed utilizing a transitional model (TIM 012032) with 1,000 bootstraps using the software PhyML (Guindon et al., 2010). The NCBI BLAST tool³ was used to compare sequences from individual isolates to the NCBI database.