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Determination of metronidazole and 2-hydroxymetronidazole in murine plasma
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Metronidazole and 2-hydroxymetronidazole were determined according to the method described earlier [16]. To each sample, internal standard (paracetamol) was added. Subsequently, 100% methanol was used to denature plasma proteins. Samples were then centrifuged, and supernatant was transferred to 1.5 mL Eppendorf tube and evaporated under nitrogen flow at 40°C. The samples were dissolved in mobile phase and applied into a Li-Chrospher RP-18 column (Merck). For HPLC analysis Shimadzu LC-20 HPLC system (Shimadzu) with UV/fluorescence detection was used.
Copyright and License information: ©2021 Zemanová et al ©2021 Hans-Joachim LehmlerThis is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ©2021 Gianfranco D. AlpiniThis is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ©2021 Gianfranco D. AlpiniThis is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ©2021 Gianfranco D. AlpiniThis is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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