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WT and B.1.351 Spike Proteins, Human ACE2 Receptor, and Antibodies

VY Victor Yin
SL Szu-Hsueh Lai
TC Tom G. Caniels
PB Philip J. M. Brouwer
MB Mitch Brinkkemper
YA Yoann Aldon
HL Hejun Liu
MY Meng Yuan
IW Ian A. Wilson
RS Rogier W. Sanders
MG Marit J. van Gils
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The 2P-stabilized S proteins of the Wuhan strain (WT) and B.1.351 variant were described previously.12,55 The B.1.351 construct contained the following mutations compared to the WT variant (Wuhan Hu-1; GenBank: MN908947.3): L18F, D80A, D215G, L242H, R246I, K417N, E484K, N501Y, D614G, and A701V. Both S constructs were produced in HEK293F suspension cells (ThermoFisher) and purified as previously described.12 For the human ACE2 receptor, soluble ACE2 was generated as described previously12 by using a gene encoding amino acids 18–740 of ACE2. The IgGs and Fab fragments used in this study were produced as previously described.12,26

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