Optimization of TH-NP

CF Camila Folle
AM Ana M. Marqués
ND Natalia Díaz-Garrido
ME Marta Espina
ES Elena Sánchez-López
JB Josefa Badia
LB Laura Baldoma
AC Ana Cristina Calpena
MG Maria Luisa García
ask Ask a question
Favorite

TH-NP were optimized using the design of experiments approach (DoE). A full factorial central design of five levels and three factors was applied in order to reduce the number of experiments [46]. This experimental design consisted of 16 formulations with variable factorial points (− 1/ + 1), axial points (− 1.68/ + 1.68) and central points (0), each involving 8, 6 and 2 formulations, respectively. The concentration of active (TH), surfactant (TW) and the pH of the aqueous phase were selected as the independent variables (Table (Table5).5). PLGA was fixed to 9 mg/mL for the entire experiment. The effect of the independent on the dependent variables (morphology, z-potential and encapsulation efficiency) has been analyzed [47].

Factors and levels of DoE independent variables

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A