Cell proliferation ability was examined by CCK-8, colony formation, EdU and cell cycle assays. CCK-8 assay was conducted using a CCK-8 assay kit (Dojindo Japan). Ethynyl deoxyuridine (Edu) assay were conducted with EdU detection kit (KeyGEN BioTECH, Nanjing, China). Colony formation assays were performed according to standard protocols [29]. The FITC-Annexin V and propidium iodide (PI) double staining apoptosis assays were performed with flow cytometer (FACScan; BD Biosciences, USA).
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