Cell proliferation and apoptosis assays

HZ Hao Zhou
WL Wei Liu
YZ Yongming Zhou
ZH Zhenya Hong
JN Jian Ni
XZ Xiaoping Zhang
ZL Ziping Li
ML Mengyuan Li
WH Wenjuan He
DZ Donghua Zhang
XC Xuexing Chen
JZ Jianhua Zhu
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Cell proliferation ability was examined by CCK-8, colony formation, EdU and cell cycle assays. CCK-8 assay was conducted using a CCK-8 assay kit (Dojindo Japan). Ethynyl deoxyuridine (Edu) assay were conducted with EdU detection kit (KeyGEN BioTECH, Nanjing, China). Colony formation assays were performed according to standard protocols [29]. The FITC-Annexin V and propidium iodide (PI) double staining apoptosis assays were performed with flow cytometer (FACScan; BD Biosciences, USA).

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