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Pathological tissue sampling
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On the day after the MRI scan was completed, the rat was killed by injecting potassium chloride into the tail vein. The heart was removed and washed with saline, then the myocardial tissue was fixed in 10% formaldehyde, embedded in paraffin, sectioned and stained with conventional hematoxylin-eosin (HE). The pathological changes in the myocardial tissue of the right ventricle were observed under an optical microscope.
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