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Immunohistochemistry was performed on 5 µm-paraffin sections with an indirect method (EnVison) using the primary antibody against MYC (Abcam, Cambridge, MA, USA, 1:100), BCL-2 (Abcam, 1:100), BCL-6 (Abcam, 1:100), and anti-rabbit/rat-IgG antibody (Dako, Carpinteria, CA, USA) as the second antibody. BCL-2, BCL-6, and MYC positives were determined as previously reported (18-21). Interpretation of immunostains was performed by two certified hematopathologists.
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