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In vitro expansion and culture of Tregs
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The isolated and purified Treg cells were cultured in a basal medium, and human AB serum (5% by volume), IL-2 (1,000 U/mL), and rapamycin (100 nM) were added; on day 1 and day 14, CD3/CD28 Dynabeads was for stimulation. Culture medium was added every 2 to 3 days, and the culture period was 21 days.
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