Cell Culture, Reagents, And Transfections

ATCC (STR profiling) authenticated bNHL cells, SUDHL2, SUDHL4, SUDHL10, and OCI-LY19. Raji was grown in the RPMI 1640 medium with 10% heat-inactivated fetal bovine serum (FBS) and 200 U of penicillin/streptomycin (Mediatech, Manassas, VA) under 5% CO₂ and at 37°C. Primary DLBCL tumor cells were obtained through Tufts Tumor Repository at Tufts Medical Center (Boston, MA) as de-identified discarded specimens through an exempt institutional review board approval. The FASN inhibitors cerulenin (Sigma Aldrich, St. Louis, MO), TVB3166, and TVB3567 were generous gifts from 3V Biosciences (Menlo Park, CA). Standards from quantitative mass spectrometry nucleotides, 6-phosphogluconate and glucose-6-phosphate, were purchased from Sigma (St. Louis, MO). RNA interference experiments were performed using FASN siRNA Ambion Cat# 4390824 (Thermo Fisher Scientific, Austin, TX) and PGDH shRNA, Mission shRNA clone TRCN0000274974 (Sigma Aldrich, St. Louis, MO).