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DNA Quantification
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DNA content was determined using the Quant-iT PicoGreen DNA assay (Invitrogen, Life Technologies, United Kingdom). Briefly, 10 μL of cell lysate (in 0.1% Triton™ X-100) was added to 90 μL of TE (10 mM Tris-HCl, 1 mM EDTA) buffer. 100 μL of PicoGreen reagent was added to all samples for 5 min. The fluorescence was then measured in a SPARK spectrophotometer (TECAN, CH) at 480/520 nm wavelength.
Copyright and License information: ©2021 Man, Brunet, Louth, Robinson, Fernandez-Rhodes, Williams, Federici, Davies, Hoey and Cox.
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