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Alkaline Phosphatase Activity
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ALP activity was determined using the 4-nitrophenyl colourimetric phosphate liquid assay (pNPP, Sigma-Aldrich, United Kingdom) as previously reported (Man et al., 2021b). Briefly, 10 μL of cell lysate (in 0.1% Triton™ X-100) was added to 90 μL of pNPP and incubated for 60 min at 37 C. The absorbance at 405 nm was read on a SPARK spectrophotometer (TECAN, CH). ALP activity was normalised with DNA content.
Copyright and License information: ©2021 Man, Brunet, Louth, Robinson, Fernandez-Rhodes, Williams, Federici, Davies, Hoey and Cox.
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