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2.3 Luciferase Reporter Assay and Plasmid Constructs

SM Sarah Morice
GD Geoffroy Danieau
RT Robel Tesfaye
MM Mathilde Mullard
RB Régis Brion
MD Maryne Dupuy
BO Benjamin Ory
BR Bénédicte Brounais-Le Royer
IC Isabelle Corre
FR Françoise Redini
FV Franck Verrecchia
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Transient cell transfections were performed with jetPEI™ (Polyplus transfection, Illkirch, France). The pRLTK-Renilla luciferase expression vector was co-transfected in all experiments to monitor transfection efficiencies. Luciferase activity was determined with the dual-Luciferase reporter assay system (Promega, Charbonnieres, France). The (CAGA)9-Lux was used as reporter constructs specific for Smad3/4-driven signaling (18). The 8xGTIIC-Luc (gift from Stefano Piccolo, Addgene plasmid #34615; http://n2t.net/addgene:34615; RRID : Addgene-34615; (TEAD)8-lux in the text) construct was used as a reporter construct specific for TEAD-driven signaling.

Copyright and License information:  ©2021 Morice, Danieau, Tesfaye, Mullard, Brion, Dupuy, Ory, Brounais-Le Royer, Corre, Redini and Verrecchia
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