Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
Cell viability was detected using Cell Counting Kit-8 (CCK-8, Dojindo, Japan) assays according to the manufacturer’s instructions. Briefly, the cells were seeded into a 96-well plate at a density of 5,000 cells/well. After treatment with different drugs at various concentrations for the indicated times, 10 µL of CCK-8 reagent was added to each well, and the cells were cultured for another 2 h. At the end of the incubation, the absorbance at 450 nm was analyzed with a microplate reader (BioTek, United States). All experiments were performed in triplicate.
Copyright and License information: ©2021 Ye, Wu, Cai, Sun, Deng, Liang and Han.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
