Total RNA Extraction and qRT-PCR Verification

Total RNA was extracted by using TRIzol (Invitrogen, United States) according to the manufacture’s protocol. cDNA was generated using a PrimeScript RT Reagent kit (TaKaRa, Japan). Real Time PCR was performed in a CFX96 Real-Time PCR Detection System (Bio-Rad, United States) using a SYBR Green Real-Time PCR kit (TaKaRa, Japan). The primer sequences used were as follows: SRC, (forward) 5’- GGC​TCC​AGA​TTG​TCA​ACA-3’ and (reverse) 5’- GCT​TGC​GGA​TCT​TGT​AGT-3' GAPDH, (forward) 5’-ATC​AAT​GGA​AAT​CCC​ATC​ACC​A-3’ and (reverse) 5’-GAC​TCC​ACG​ACG​TAC​TCA​GCG-3’. Relative mRNA values were normalized to the expression of the GAPDH gene using the 2^−∆∆Ct method.