Reagents and antibodies

TH Takaya Handa
KM Keita P. Mori
AI Akira Ishii
SO Shoko Ohno
YK Yugo Kanai
HW Haruko Watanabe-Takano
AY Akihiro Yasoda
TK Takashige Kuwabara
NT Nobuyuki Takahashi
NM Naoki Mochizuki
MM Masashi Mukoyama
MY Motoko Yanagita
HY Hideki Yokoi
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Adriamycin was obtained from Sigma Aldrich (St. Luis, MO). FR167653, p38α MAPK inhibitor, was kindly provided by Astellas Pharma Inc. (Tokyo, Japan). Primary antibodies used for immunohistochemical studies and Western blotting were mouse anti-NPR-C (1:100, sc-515449; SantaCruz, Dallas, TX), goat anti-nephrin (1:100, AF3159; R&D Systems, Minneapolis, MN), rabbit anti-monocyte chemotactic protein-1 (1:100, ab25124; Abcam, Cambridge, UK), rabbit anti-phospho-p38 MAPK (1:1000, #9211; Cell Signaling Technology, Boston, MA), mouse anti-desmin (1:150, M0760; DAKO, Tokyo, Japan), mouse anti-β actin (1:1000, A5411; Sigma-Aldrich), and rat anti-MAC-2 (1:100, CL8942F; Cedarlane, CA).

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