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Our team centrifuged cells that treated to remove the supernatant, which were fixed with fixation solution (4% paraformaldehyde, 0.25% glutaraldehyde in 0.1 M phosphate buffer pH 7.4) at 4 °C over the night. The cells were treated following the protocol of Fujian Medical University (Fuzhou, China). We observed ultrathin sections under transmission electron microscope (Quanta 450, FEI, USA).
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