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Exome sequencing libraries were prepared by using a Nextera Rapid Capture Kit (Illumina, Calif, USA) based on the manufacturers’ protocol with slight modifications. The library concentration was quantified by a Qubit dsDNA Broad Range Assay Kit (Invitrogen, USA). Library size was measured by using a LabChip 3 K Hisense Kit (PerkinElmer, USA). Paired-end exome sequencing with 150 bp cycles was performed on a HiSeq 4000 (Illumina, Calif, USA), targeting an averaged depth of 100X.
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