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Cells plated on LabTek™ chamber slides (Thermo Fisher Scientific) were treated with CSK buffer and/or Benzonase nuclease, washed with PBS, and fixed with 4% paraformaldehyde for 20 min at room temperature. After washing, cells were mounted using ProLong Gold antifade reagent (Vector Laboratories, Burlingame, CA, USA). Confocal images were acquired using an LSM880 confocal microscope (Carl Zeiss) with a 40 × /1.2 objective lens. Image acquisition and analysis were performed with Zen 2.6 (blue edition) (Carl Zeiss) software.
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