Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
The viability of tacrolimus-treated kidney organoids within a kidney organoid-on-a-chip was analyzed by using a live/dead assay after 6 days. (Thermo Fisher Scientific, MA, USA). Briefly, 5 mL of DPBS containing 2 µL of calcein AM solution and 10 µL of ethidium homodimer-1 solution was added to kidney organoid-on-a-chip and was then incubated at 37 °C in a 5% CO2 incubator for 40 min. The stained organoids were analyzed by using an inverted fluorescence microscope (Olympus, Japan).
Copyright and License information: The Author(s) ©2021
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
