Quantification and statistical analysis

AS Aleksandra Szykowska
YC Yu Chen
TS Thomas B. Smith
CP Charlotta Preger
JY Jingjing Yang
DQ Dongming Qian
SM Shubhashish M. Mukhopadhyay
EW Edvard Wigren
SN Stephen J. Neame
SG Susanne Gräslund
HP Helena Persson
PA Peter J. Atkinson
ED Elena Di Daniel
EM Emma Mead
JW John Wang
JD John B. Davis
NB Nicola A. Burgess-Brown
AB Alex N. Bullock
ask Ask a question
Favorite

All statistical analyses were carried out using three independent biological replicates and two or three technical repeats unless specifically stated otherwise. Error bars are standard deviation (SD) and EC50 was interpolated using non-linear regression (inhibitor vs. response- four parameters) in GraphPad Prism Software 8.4.2. Each statistical test was performed on raw data normalised to cell number or signal before normalisation to the percentage of control was performed. One-way ANOVA with Dunnett’s test comparison to control untreated cells was utilised to calculate significance. p values are indicated in the respective figure legends with symbols (P <.05 and ∗∗P <.01; ∗∗∗∗P <.0001).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A