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Transfected THP-1 and HL-60 cells were seeded into 6-well plates (1000 cells/well) and cultured for approximately 12 days at 37°C to allow colony growth. The colonies were fixed with 4% paraformaldehyde, stained with 0.1% crystal violet and photographed. The number of colonies was counted under a microscope (Olympus, Tokyo, Japan).
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