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Labeling RNA and FACS analysis.

WN Wolfgang Nacken
AS André Schreiber
DM Dörthe Masemann
SL Stephan Ludwig
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Cellular RNA was labeled by adding ethynyl uridine (EU) to the medium. Subsequently, the cells were fixed and permeabilized with 4% paraformaldehyde and 0.1% Triton X-100. Chemical labeling of the EU with a fluorophore-containing RNA was performed by click chemistry according to the manufacturer’s protocol (Jena Bioscience, Germany). Cells were analyzed by FACS analysis according to standard procedures.

Copyright and License information:  ©2021 Nacken et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

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