E. histolytica trophozoites (HM1: IMSS) were cultured according to the Diamond Method [25], while E. dispar (SAW 760 strain) and E. invadens (IP-1; ATCC 30994) trophozoites were maintained in LYI-S-2 medium TYI-S-33 and incubated at 37°C and 28°C, respectively [26].
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