2.1. Culturing the Parasites

AN Abril Navarrete-Mena
JP Judith Pacheco-Yépez
VH Verónica Ivonne Hernández-Ramírez
AE Alma Reyna Escalona-Montaño
JG Jenny Nancy Gómez-Sandoval
MN Mario Néquiz-Avendaño
BC Bibiana Chávez-Munguía
ET Emiliano Tesoro-Cruz
PT Patricia Talamás-Rohana
MA María Magdalena Aguirre-García
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E. histolytica trophozoites (HM1: IMSS) were cultured according to the Diamond Method [25], while E. dispar (SAW 760 strain) and E. invadens (IP-1; ATCC 30994) trophozoites were maintained in LYI-S-2 medium TYI-S-33 and incubated at 37°C and 28°C, respectively [26].

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