4.3. MTT Cell Viability Assay

Cells were treated (see Methods above) in duplicate using increasing concentrations of DMC or EF24. Plate was incubated for 72 h at 37 °C, 5% CO₂. MTT (Thermo Fisher, Dublin, Ireland) solution was diluted using pre-warmed cell media to a working concentration of 0.5 mg/mL and 500 µL of working MTT solution was added to each well after old medium was removed. The plate was incubated in the dark for 2 h at 37 °C. The MTT solution was discarded and 500 µL of DMSO was added to each well and plate was incubated for 1h at room temperature. Absorbance was read at 570 nm using CLARIOstar plate reader (BMG Labtech, Ortenberg, Germany).