Tomato seeds (Solanum lycopersicum cv. Floradade, Crown Seeds, Homestead, FL, USA) were germinated in peat moss until 3–4 leaves were presented. Then, tomato seedlings were transplanted into 1.5 L pots containing peat moss and perlite (70:30 v/v). The plants were watered every three days and fertilized once a week with 20N-20P-20K and microelements solution (FertiDrip, Agrodelta, Monterrey, Mexico). All plants were maintained under greenhouse conditions at 28 ± 2 °C with relative humidity (RH) = 45% and regularly monitored until they reached 6 to 7 fully extended leaves before bioassays.
The tomato psyllids (Bactericera cockerelli) CaLso-positive were reared on tomato plants under greenhouse conditions [31] to induce infestation. Control plants were kept separated to avoid infestation.
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