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Three slides from each muscle group of each animal were stained with an immuoflorescent anti-CD31 antibody, using a standard protocol, as a marker of endothelial cells and, consequently, angiogenesis. Staining involved antigen retrieval and inhibition of endogenous peroxidase activity prior to incubation with the primary antibody (rabbit polyclonal to CD31) and secondary antibody (goat anti-rabbit IgG).
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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