4.3. TNBS Induced Colitis Model

Before establishing the trinitro-benzene-sulfonic acid (TNBS) induced colitis model, 8-week-old mice were dermally pre-sensitized using 150 μL 1% TNBS C57BL/6. A week later, 100 μL 2.5% TNBS was delivered rectally. Oral delivery of Fexofenadine (0.4, 2, 10 mg/kg), Terfenadine (2, 10, 50 mg/kg), 5-aminosalicylic acid (5-ASA, 50 mg/kg, serving as a positive control) were initiated three days before model initiation and continued daily for 5 days. Body weights were recorded daily. After sacrifice, colon length was measured, and colons and the sera were processed for further analysis. Six mice were used for each group.