4.8. EtOH/HCl-Induced Gastritis Mouse Model

Five-week-old male ICR mice (5 mice/group) were orally treated with Pd-EE (100 mg/kg) or ranitidine (40 mg/kg) three times per day for two days. At the third day, the gastritis was induced with EtOH/HCl (300 μL of 60% ethanol in 200 mM HCl) at 8 h after the final oral injection. The mice were anesthetized and sacrificed using isoflurane, and their stomachs were extracted. The stomachs were washed with PBS several times and photographed. Photographs of the injured lesions were analyzed using ImageJ software (ImageJ bundled with 64-bit Java 1.8.0_172). Then, the stomach tissue was ground with liquid nitrogen and stored at −70 °C until they were used. When used in western blotting assays, the tissues were lysed in lysis buffer, and when mRNA levels were checked, they were lysed by TRIzol reagent. The approval number received for this experiment is as follows (SKKUIACUC2020-09-48-1).