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4.5. In Vitro Monocyte Migration Assay

ÁP Álvaro Petersen-Uribe
MK Marcel Kremser
AR Anne-Katrin Rohlfing
TC Tatsiana Castor
KK Kyra Kolb
VD Valerie Dicenta
FE Frederic Emschermann
BL Bo Li
OB Oliver Borst
DR Dominik Rath
KM Karin Anne Lydia Müller
MG Meinrad Paul Gawaz
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Monocyte migration was analyzed in a modified 48-well Boyden chamber (Neuro Probe Inc., Gaithersburg, MA, USA) as described [23]. Monocytes were added in the upper compartment, and recombinant human PCSK9, evolocumab, supernatant obtained from resting (RPS), or activated platelets (APS) were added to the lower compartment in concentrations as indicated. After four hours, monocytes associated with the filter membrane were detected by May–Grünwald/Giemsa staining (Merck Millipore, Darmstadt, Germany). Migrated monocytes on the filter were detected using a microscope (Nikon Eclipse Ti2-A, Nikon, Tokyo, Japan).

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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