2.4.3. Hypertension Induction (Goldblatt Model; Two Kidneys, One Clip; 2K1C)

After the anesthesia by intraperitoneal injection of ketamine at 100 mg/kg and xylazine 20 mg/kg, the male rats were submitted to laparotomy, the left renal artery was isolated, and a silver clip (1.5 mm lumen) was placed to partially limit blood flow [18]. Systolic blood pressure (SBP) was weekly measured by the tail-cuff method. Only animals with SBP above 140 mm Hg were used in experiments.