4.8. Measurement of Transepidermal Water Loss, Stratum Corneum Hydration

JS Jung Eun Seol
SA Sang Woo Ahn
BS Bomin Seol
HY Hyeong Rok Yun
NP Nammi Park
HK Hyoung Kyu Kim
EV Elena A. Vasileva
NM Natalia P. Mishchenko
SF Sergey A. Fedoreyev
VS Valentin A. Stonik
JH Jin Han
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The day after the completion of every 2 week schedule of UV irradiation, the transepidermal water loss (TEWL) and stratum corneum hydration (SCH) were measured by a tewameter and corneometer using a Cutometer™ MPA 580 (Courage and Khazaka Electronics™, Cologne, Germany). A measuring probe was placed on the middle portion of the dorsal skin of the mice, which were anesthetized by inhaling a low concentration of isoflurane. Values of TEWL were read at the plateau of the estimation graph, while SCH values were taken as the mean value of three measurements.

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